Transfer Membrane

Porablot NCP membrane filters are the classical transfer medium for binding proteins and nucleic acids. Applications include Southern, Northern and Western blotting, as well as colony, plaque transfer and dot blotting. Although the binding mechanism is not understood in all details, it is assumed that the macromolecules are bound via hydrophobic interaction and hydrogen bonding. The membranes consist of 100% nitrocellulose and are supplied with a pore size of 0.45 µm. With a binding capacity of 100 µg/cm, this membrane is well suited for protein blotting. The hydrophilic character of this membrane ensures good contact with the gel during blotting. Additionally, nitrocellulose can be easily blocked (e.g. with casein or albu-min 3). Due to the low background, all conventional detection methods, such as antigen / antibody, autoradiography, biotin labeling and chemoluminescence, yield good results.

Cat. No.	Size	Pack of
741280	0.3 x 3 m	1 roll
741281	200 x 200 mm	10 sheets
741282	Ø 132 mm	50 disks
741283	Ø 82 mm	50 disks

MACHEREY-NAGEL, Inc., 6 South Third Street, Suite 402, Easton, PA 18042. Tel: 610-559-9848; Fax: 610-559-9878.