Product/Service

Prime RNase Inhibitor

Source: Eppendorf Scientific, Inc.
Prime RNase Inhibitor is a protein of non-human origin that inhibits the same types of ribonuclease as Human Placental RNase Inhibitors (HPRI)
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Prime RNase Inhibitor is a protein of non-human origin that inhibits the same types of ribonuclease as Human Placental RNase Inhibitors (HPRI) , including RNases A, B and C. Prime RNase Inhibitor can be used in cDNA synthesis, in in vitro transcription using the SP6, T7 and HeLa cell extract systems and in in vitro translations using rabbit reticulocyte lysates and wheat germ extracts. Additionally, Prime RNase Inhibitor prevents RNA degradation in cell extracts prepared under non-denaturing conditions, and can be used in first strand synthesis prior to PCR (RT-PCR). Prime RNase Inhibitor can also be used in gel shift assays , the isolation of an RNA binding protein by oligonucleotide affinity purification, nuclear run-on transcription assays, and in polysome distribution analysis at +4°C.

Other Features

Inhibits greater than 90% of RNase activity where Human Placental
RNase Inhibitors (HPRI) inhibits only 50%
Stable under a broad range of pH, DTT concentrations and
temperatures
Inhibits RNases A, B and C, just like HPRI, and does not interfere
with RNases T1, T2, H, U1, U2 or CL3

Eppendorf Scientific, Inc., One Cantiague Road, Westbury, NY 11590-0207. Tel: 800-421-9988; Fax: 516-876-8599.