The corneal irritancy of test materials is known to be related to their propensity to promote denaturation and disruption of corneal proteins. The Ocular Irritection Assay System is an in vitro test method that mimics these biochemical phenomena. This test consists of two essential components:
Controlled mixing of the test material and the reagent solution during the assay incubation period promotes protein denaturation and disaggregation of the macromolecular matrix.
The changes in protein structure that are induced by the test material may be readily quantitated by measuring the resulting changes in turbidity (OD405) of the reagent solution.
Comparison of these optical density measurements to those produced by standard chemical irritants permits calculation of an "irritancy score" that has been shown to be directly related to the potential corneal irritancy of the test material.
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