Cryptosporidium Stain Kit (Modified Kinyoun Method)
This technique aids in differentiating Crytosporidium oocysts from yeast cells.
RECOMMENDED PROCEDURE: Specimen may be prepared from fresh material or preserved in 10% buffered formalin (Product # 9700). Formalin-preserved Specimens: Mix stool suspension thoroughly and filter an adequate amount through cheesecloth into a centrifuge tube. Spin for 2 minutes, decant supernatant and add saline to the remaining sediment. Centrifuge again and decant saline. Smears may be prepared from upper layer of sediment. Material should be spread on a clean glass slide and allowed to air dry. Fix fresh specimens in methanol and formalized specimens with heat. Flood smear with Sol. I for 1 - 2 minutes. Rinse gently with water. Decolorize with Sol. 11 and rinse again with water. Counterstain with Sol. III for 30 - 60 seconds. Rinse with water and allow to dry. NOTE: Decolorization and counterstaining times may vary according to thickness of specimen.
RESULTS: Cryptosporidium oocysts - light pink to deep red; yeast cells - green to blue-purple.
CONTENTS: Basic Fuchsin, Ethyl Alcohol, Phenol, Glacial Acetic Acid, HCL, Light Green SF
These solutions are made from certified dyes (when applicable). For in vitro diagnostic use only.
ENG Scientific, Inc., 82 Industrial East, Clifton, NJ 07012. Tel: 973-472-7200; Fax: 973-472-9460.