Product/Service

Column Backflushing

Source: IDEX
Column backflushing is an analytical method in which a fluid switching platform reverses the flow through the column. This allows automatic clean up of the column without disconnection in situations where sample matrix is trapped on the head of the column

Column backflushing is an analytical method in which a fluid switching platform reverses the flow through the column. This allows automatic clean up of the column without disconnection in situations where sample matrix is trapped on the head of the column. The reversed flow permits the compounds to be eluted in a shorter period of time rather than force the highly retained compound through the full length of the column.

For automated column backflushing, connect a Two-Position, Six-Port platform, used as a switching platform, to the system in place of the column between the autosampler (or injector) and the detector. The illustration shows the column connecting to the Two-Position, Six-Port platform and a jumper tube to enable flow reversal. Simply switching the platform changes the direction of flow through the column only. Choosing the appropriate tubing size is an important factor to minimize volume added to the system that could increase dispersion.