Centri·Sep
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri·Sep unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.
The Centri·Sep gel will provide excellent recovery of DNA fragments with sizes greater than 16 base pairs or 25-mer, while removing > 98 % of salts, NTP's and other unwanted low- molecular- weight impurities.
The column gel is hydrated with either reagent-grade water or a suitable buffer or your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low- molecular- weight components, but also exchanged into the buffer of your choice.
Princeton Separations, Inc., P.O. Box 300, Adelphia, NJ 07710. Tel: 732-431-3338; Fax: 732-431-3768.