Alternating Column Regeneration

Alternating column regeneration using two matched columns can increase sample throughput by equilibrating one column while the other column is separating the sample. An automated fluidic switching platform enhances the convenience of column regenerating by switching between the analyzing column and the equilibrating column.

For example, in an analysis with a total analysis time of 30 minutes, 20 minutes might be required for the analysis and 10 minutes for equilibration to prepare for the next sample. Using alternating column regeneration, the 10-minute regeneration time occurs during analysis, reducing the total analysis time to 20 minutes. This technique reduces analysis time in this example by one-third. Time saved will depend on the column equilibration time.

Column regeneration is beneficial for laboratories running large quantities of samples such as for drug discovery and quality assurance. This application increases efficiency and saves money for repetitive chromatographic analyses. Rheodyne's Two-Position, Ten-Port switching platform makes unattended operation of this technique possible without the wait of a long equilibration time.