White Paper

Optimizing Ideal Conditions For Liquid Chromatography Systems

Source: Sartorius

By Gregory Krueger, Kevin Epting, Priyanka Gupta, and Roger Alsop, Sartorius Stedim Biotech

Sartobind® Pico and Sartobind® Nano are the laboratory-scale members of the Sartobind® membrane adsorber family. Sartobind® Pico capsules are especially suited for the removal of DNA, viruses and protein contaminants in biopharmaceutical production. The large pores (>3 um) increase the process speed and avoid diffusion limits compared to conventional bead chromatography. Membrane adsorbers feature extremely short cycle times and provide exceptionally high flow rates and throughput.

Sartobind® Jumbo capsules are ion exchange chromatography devices based on macroporous membranes. They can be used for chromatographic separations in the downstream processing of proteins. The ion exchange ligands are coupled to a membrane which is fitted into a plastic housing for quick handling, making ion exchange purification nearly as easy as filtration. Jumbo capsules are constructed with optimized fluid channels. They carry an internal central core to minimize void volume.

While both the Pico and Nano capsules are designed for high flow rates, the array of valves, pumps,  monitors and the flow restrictor found in a typical flow path for gel chromatography can cause unacceptable pressures at the designed flow rates of the capsules. Although a lower flow rate will reduce the pressure in the system, this does not take advantage of the rapid processing offered by Sartobind® membrane adsorbers.

A method designed to maintain ideal conditions without generating excessive pressure is described along with an alternative Flow Path configuration for a typical Akta® system.