You have identified a need for a point of care detection assay, you know what your test medium is, what you are hoping to detect, and a lateral flow assay (LFA) fits the bill. You may even have developed an LFA to meet the initial need. But there’s still something missing. What if identifying multiple targets could vastly improve detection, sensitivity and specificity, or perhaps there are multiple causes or markers you wish to screen for simultaneously?
There are numerous reasons why combining detection of multiple targets within a single assay is desirable. This guide outlines some of the important factors to consider when developing a multiplex LFA, be it from scratch or building on previously developed singleplex assays.