Application Note

Fast Separation Of Triacylglycerols In Oils Using UltraPerformance Convergence Chromatography (UPC2)

Source: Waters Corporation

By Mehdi Ashraf-Khorassani, Larry T. Taylor, Jinchuan Yang, and Giorgis Isaac

Studies using UPC2 coupled with ultraviolet (UV) detection, mass spectrometry (MS), and evaporative light scattering (ELS) detection for the separation of triacylglycerols in tobacco, corn, sesame, and soybean seed oils are presented in this application note.

UltraPerformance Convergence Chromatography, in combination with sub-2-μm particles and UV, ELS, and MS detection, is a valuable technique for the determination of triacylglycerol composition in a variety of seed oils. Excellent resolution on a single column in as little as 10 minutes serves as an improvement on past generations of SFC instrumentation. This methodology can be used as a tool for rapid characterization and profiling a suite of acylglycerols from different oil sources.