By Jonathan P. Danaceau, Kenneth J. Fountain, and Erin E. Chambers, Waters Corporation
This application note uses a panel of natural and synthetic opioid drugs to demonstrate the use of a systematic screening strategy for UPC2 method development. The simultaneous evaluation of multiple column chemistries and several different mobile-phase additives allows rapid determination of preferred starting conditions for further optimization. This enabled easy and rapid optimization of conditions to achieve a method capable of analyzing 19 different opioid drugs in less than 2 minutes, with acceptable retention and peak shape for all compounds. In addition, this method further highlights the potential utility of UPC2 for the analysis of a wide variety of compound classes.