Application Note

Removal Of Endotoxin From Monoclonal Antibodies

Source: Sartorius

It is desirable to minimise endotoxins in purified protein preparations prior to their use in cell-based assays. Vivapure® centrifugal anion exchange membrane devices can remove endotoxin from research grade monoclonal antibody solutions simply with high protein recovery.

Endotoxins are lipopolysaccharides present in the cell wall of most Gram-negative bacteria, and are frequently present as contaminants in protein solutions purified in research environments. They have profound biological effects and thus must be minimised prior to use of such preparations in cell-based assays. The term EU is used to describe the activity of endotoxins, and typically the limit for endotoxin is set at 50 EU/mg for bioactive proteins destined for cell-based assays.

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