Pipetting accuracy and precision are the key factors for successful experiments in the laboratory. The best pipetting performance is achieved, when the pipette and the tip match perfectly with each other, and the tip being used is optimal for the liquid being transferred.
When pipetting liquids with low surface tension (e.g. detergents), they tend to leave a film of liquid on the inner surface of standard polypropylene pipette tips, leading to inconsistency and inaccuracy in pipetting as well as loss in volume of valuable samples.
Various technologies are being used to modify the standard pipette tips to mitigate this issue. This application note describes the comparison of various manufacturers’ low retention tips, when handling liquids that contain detergents. The results show that Sartorius Low Retention Tips ensure near complete recovery of the sample, when pipetting liquids of low surface tension. When comparing to other low retention tips from various manufacturers, Sartorius tips secured the best sample recovery and the best chemical resistance.