Application Note | August 12, 2014

Lipid Class Separation Using UPC2/MS

Source: Waters Corporation
By Michael D. Jones, Giorgis Isaac, Giuseppe Astarita, Andrew Aubin, John Shockcor, Vladimir Shulaev, Cristina Legido-Quigley, and Norman Smith

A flexible, universal method was developed for the analysis of inter-class separations of neutral and amphipathetic lipids. The organic phase of the biological lipid extract can be directly injected onto the ACQUITY UPC2 System with MS detection, thereby saving time and operating costs. The rapid inter-class screening provided an analysis within seven minutes including re-equilibration.

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