By Marian Twohig, Andrew Aubin, Michael O’Leary, Tom DePhillipo, Sherry C. Perine, and David R. Stubbs
In this application note we have shown the analysis of chiral pesticides using ACQUITY UPC2. ACQUITY UPC2 allows high efficiency separations that can significantly increase the sample throughput when compared with traditional normal-phase separations. 10 The time taken to develop a method from the column and co-solvent screening step to the final optimized method is decreased. The methods described here use supercritical CO2 as the primary mobile phase and predominantly 2-propanol as the organic modifier. The need to use large volumes of potentially hazardous solvents is reduced as is the cost associated with solvent waste disposal. The %RSD’s obtained were comparable to those obtained by UPLC®/UV methods.
The study of enantioselective toxicity and environmental fate has previously been a challenge due to the difficulty in resolving chiral compounds. The benefit of having faster analytical methods to resolve chiral compounds means that critical information pertaining to their stereoselective behavior can be obtained more rapidly.