Application Note

Bile Acid Profiling Using UltraPerformance Convergence Chromatography (UPC2) Coupled To ESI-MS/MS

Source: Waters Corporation
WatersCorp

By Kaori Taguchi, Eiichiro Flukusaki, and Takeshi Bamba

UltraPerformance Convergence Chromatography combined with ESI-MS/MS enabled the simultaneous profiling of 25 bile acids within 13 minutes. This novel separation method is highly suitable for determining the effects of bile acid levels in triglyceride, cholesterol, and glucose metabolism and has the potential to speed up the development of novel drug targets for metabolic diseases.